Control:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Immunohistochemistry:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
MANN-WHITNEY:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Over Expression:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Knockdown:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Immunohistochemical staining:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Flow Cytometry:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Negative Control:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Positive Control:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Injection:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Marker:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Expressing:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Enzyme-linked Immunosorbent Assay:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Western Blot:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Immunofluorescence:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Functional Assay:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
RNA Sequencing:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Modification:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
RNA Immunoprecipitation:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Sequencing:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Plasmid Preparation:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Co-Immunoprecipitation Assay:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Transfection:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Activation Assay:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Immunoprecipitation:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
In Vivo:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Isolation:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Concentration Assay:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
MTT Assay:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Incubation:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Labeling:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Electrophoresis:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Staining:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Migration:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Spectrophotometry:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Membrane:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Quantitative RT-PCR:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Irradiation:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Colony Assay:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
TUNEL Assay:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Inhibition:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Fractionation:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Binding Assay:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Luciferase:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
Reporter Assay:Article Title: NAT10‐mediated ac 4 C‐modified ANKZF1 promotes tumor progression and lymphangiogenesis in clear‐cell renal cell carcinoma by attenuating YWHAE‐driven cytoplasmic retention of YAP1
Article Snippet: Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).Written informed consent was obtained from all patients, and the present study was authorized by the ethics committee of Union Hospital (IEC‐072).. Human embryonic kidney 293T (HEK293T) cells, renal proximal tubule epithelial cells (RPTEC), ccRCC cells (A498, 786‐O, CAKI‐1, and OSRC‐2), and lymphatic endothelial cells (HLECs) were purchased from the American Type Culture Collection (Manassas, VA, USA).. These cell lines were confirmed by short tandem repeat profiling in August 2021.These cell lines were confirmed by short tandem repeat profiling in August 2021.
Article Title: DAB2IP stabilizes p27 Kip1 via suppressing PI3K/AKT signaling in clear cell renal cell carcinoma.
Article Snippet: ccRCC cells (A498, Caki-2, ACHN, and 786-O) were all purchased from the American ATCC cell bank.. Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.Caki-2 and 786-O cells were maintained in RPMI1640 medium (Gibco, Santa Clara, CA) supplied with 10% fetal bovine serum (FBS), ACHN and A498 were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplied with 10% FBS.
Article Title: Long non-coding RNA MEG8 induced by PLAG1 promotes clear cell renal cell carcinoma through the miR-495-3p/G3BP1 axis.
Article Snippet: All ccRCC cells (A498, Caki1, 786-O, 769-P, and ACHN) and normal renal cell HK-2 were purchased from American Type Culture Collection.. McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.McCoy’s 5A Medium was applied to culture Caki1 cells, and Roswell Park Memorial Institute-1640 (RPMI-1640) was used to culture other cells supplied with 10% FBS (Gibco, USA) in a 37 ◦C environment with 5% CO2.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO 2 atmosphere at 37 °C.
Article Title: LINC01094 triggers radio-resistance in clear cell renal cell carcinoma via miR-577/CHEK2/FOXM1 axis.
Article Snippet: Human kidney proximal tubule cell (HK-2), human embryonic kidney cell (HEK-293T) and ccRCC cells (A-498, ACHN, 786-O, Caki-1) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.All cells were cultivated in RPMI-1640 medium (Invitrogen, Carlsbad, CA, USA) adding 10% fetal bovine serum (FBS; Invitrogen) plus 1% penicillin/ streptomycin (Thermo Fisher Scientific, Grand Island, NY, USA) in a 5% CO2 atmosphere at 37 °C.
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